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spint2 (Sino Biological)

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Sino Biological spint2

Metavenom Kunitz type inhibitor domains can potentiate MRGPRX4. A , volcano plot of animal venom, metavenome, and secretome libraries binding to HEK293-MRGPRX4 cells versus HEK293 cells. Each point is a unique peptide in the AV, MV, and secretome libraries. B , heat map showing selectivity of fold-change values of the six peptide candidates across the HEK293-MRGPRX families. C , MSA analysis and motif discovery with MEME Suite identifies conserved amino acids shared among the candidate hits ( left ). Structural alignment of the hits using AlphaFold ( right ). D , flow cytometric analysis of ERR1712142|105-166 cell binding. MRGPRX4-overexpressing cells and parental HEK293 cells were incubated with ERR1712142|105-166 fused with a FLAG-tag. Following incubation, the cells were stained with Alexa Fluor 647-labeled anti-FLAG antibodies to quantify the amount of specifically bound candidate ligand via flow cytometry. An Empty control, which expressed irrelevant recombinant protein, was included as an additional negative control to ensure the specificity of the binding measurement. E , flow cytometric analysis of cell binding of TFPI ( left ), <t>SPINT2</t> ( middle ), and APP751 ( right ). MRGPRX4-overexpressing cells and parental HEK293 cells were incubated with TFPI, SPINT2, or APP751 fused with a His-tag. Following incubation, the cells were stained with Alexa Fluor 647-labeled anti-His antibodies. MFI, median fluorescence intensity. Data are shown as mean ± SD of 5 (in D ) or 3 (in E ) biological replicates. Statistical comparisons use two-tailed unpaired Student’s t tests. ns, nonsignificant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. F , dose-response curves of TFPI ( left ) and SPINT2 ( right ) in the presence of 200 μM UDCA using the MRGPRX4 PRESTO-Tango assay. Each point is one replicate. RLU, relative luminescence unit. G , dose-responses curves of UDCA in the presence of 0.2 μM TFPI ( left ) or 0.8 μM SPINT2 ( right ) in the MRGPRX4 PRESTO-Tango assay. Data are shown as mean ± SD of three technical replicates. MSA, multiple sequence alignment; SPINT2, serine peptidase inhibitor, Kunitz type 2; TFPI, tissue factor pathway inhibitor; UDCA, ursodeoxycholic acid.

Spint2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spint2/Human+SPINT2+Protein/pmc11833617-127-23-24
Average 92 stars, based on 1 article reviews

spint2 - by Bioz Stars, 2026-09

92/100 stars

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1) Product Images from "Molecular Display of the Animal Meta-Venome for Discovery of Novel Therapeutic Peptides"

Article Title: Molecular Display of the Animal Meta-Venome for Discovery of Novel Therapeutic Peptides

Journal: Molecular & Cellular Proteomics : MCP

doi: 10.1016/j.mcpro.2024.100901

Figure Legend Snippet: Metavenom Kunitz type inhibitor domains can potentiate MRGPRX4. A , volcano plot of animal venom, metavenome, and secretome libraries binding to HEK293-MRGPRX4 cells versus HEK293 cells. Each point is a unique peptide in the AV, MV, and secretome libraries. B , heat map showing selectivity of fold-change values of the six peptide candidates across the HEK293-MRGPRX families. C , MSA analysis and motif discovery with MEME Suite identifies conserved amino acids shared among the candidate hits ( left ). Structural alignment of the hits using AlphaFold ( right ). D , flow cytometric analysis of ERR1712142|105-166 cell binding. MRGPRX4-overexpressing cells and parental HEK293 cells were incubated with ERR1712142|105-166 fused with a FLAG-tag. Following incubation, the cells were stained with Alexa Fluor 647-labeled anti-FLAG antibodies to quantify the amount of specifically bound candidate ligand via flow cytometry. An Empty control, which expressed irrelevant recombinant protein, was included as an additional negative control to ensure the specificity of the binding measurement. E , flow cytometric analysis of cell binding of TFPI ( left ), SPINT2 ( middle ), and APP751 ( right ). MRGPRX4-overexpressing cells and parental HEK293 cells were incubated with TFPI, SPINT2, or APP751 fused with a His-tag. Following incubation, the cells were stained with Alexa Fluor 647-labeled anti-His antibodies. MFI, median fluorescence intensity. Data are shown as mean ± SD of 5 (in D ) or 3 (in E ) biological replicates. Statistical comparisons use two-tailed unpaired Student’s t tests. ns, nonsignificant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. F , dose-response curves of TFPI ( left ) and SPINT2 ( right ) in the presence of 200 μM UDCA using the MRGPRX4 PRESTO-Tango assay. Each point is one replicate. RLU, relative luminescence unit. G , dose-responses curves of UDCA in the presence of 0.2 μM TFPI ( left ) or 0.8 μM SPINT2 ( right ) in the MRGPRX4 PRESTO-Tango assay. Data are shown as mean ± SD of three technical replicates. MSA, multiple sequence alignment; SPINT2, serine peptidase inhibitor, Kunitz type 2; TFPI, tissue factor pathway inhibitor; UDCA, ursodeoxycholic acid.

Techniques Used: Binding Assay, Incubation, FLAG-tag, Staining, Labeling, Flow Cytometry, Control, Recombinant, Negative Control, Fluorescence, Two Tailed Test, Sequencing

Incubation:

Article Title: Molecular Display of the Animal Meta-Venome for Discovery of Novel Therapeutic Peptides.
Article Snippet: .. The cells were then incubated with ERR1712142 (fused with FLAG-tag), 30nM TFPI (Acro Biosystems, Cat No. TFI-H5226, fused with His-tag), 30nM SPINT2 (Sino Biological, Cat No. 10324-H08H, fused with His-tag) and 30nM APP751 (BioLegend, Cat No. 842601, fused with His-tag) prepared in 1% PBSA for 4h at 4°C on an end-to-end rotator. .. After washing the cells three times with ice-cold 1% PBSA, the cells were washed one more time with FACS washing buffer and then stained with Alexa Fluor 647-conjugated FLAG tag antibody (ThermoFisher Cat No. MA1-142-A647, 1:400 dilution) for ERR1712142 or His tag antibody (ThermoFisher Cat No. MA1-21315-A647, 1:1000 dilution) for human homologs for 10-15min at room temperature in the dark.

Article Title: Molecular Display of the Animal Meta-Venome for Discovery of Novel Therapeutic Peptides
Article Snippet: .. The cells were then incubated with ERR1712142, 30nM TFPI (Acro Biosystems, Cat No. TFI-H5226), 30nM SPINT2 (Sino Biological, Cat No. 10324-H08H) and 30nM APP751 (BioLegend, Cat No. 842601) prepared in 1% PBSA for 4h at 4°C on an end-to-end rotator. .. After washing the cells three times with ice-cold 1% PBSA, the cells were washed one more time with FACS washing buffer and then incubated with PE or Alexa647-conjugated FLAG tag antibody (PE, BioLegend Cat No. 637309, 1:150 dilution; or Alexa-647, ThermoFisher Cat No. MA1-142-A647, 1:400 dilution) for ERR1712142 or His tag antibody (ThermoFisher Cat No. MA1-21315-A647, 1:1000 dilution) for human homologs for 10-15min at room temperature in the dark.

Article Title: Molecular Display of the Animal Meta-Venome for Discovery of Novel Therapeutic Peptides
Article Snippet: .. The cells were then incubated with ERR1712142 (fused with FLAG-tag), 30 nM TFPI (Acro Biosystems, Cat No. TFI-H5226, fused with His-tag), 30 nM SPINT2 (Sino Biological, Cat No. 10324-H08H, fused with His-tag) and 30 nM APP751 (a 751 amino-acid isoform of amyloid-beta precursor protein) (BioLegend, Cat No. 842601, fused with His-tag) prepared in 1% PBSA for 4 h at 4 °C on an end-to-end rotator. .. After washing the cells three times with ice-cold 1% PBSA, the cells were washed one more time with FACS washing buffer and then stained with Alexa Fluor 647-conjugated FLAG tag antibody (Thermo Fisher Scientific, Cat No. MA1-142-A647, 1:400 dilution) for ERR1712142 or His tag antibody (Thermo Fisher Scientific, Cat No. MA1-21315-A647, 1:1000 dilution) for human homologs for 10-15 min at room temperature in the dark.

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Journal: Molecular & Cellular Proteomics : MCP

Article Title: Molecular Display of the Animal Meta-Venome for Discovery of Novel Therapeutic Peptides

doi: 10.1016/j.mcpro.2024.100901

Figure Lengend Snippet: Metavenom Kunitz type inhibitor domains can potentiate MRGPRX4. A , volcano plot of animal venom, metavenome, and secretome libraries binding to HEK293-MRGPRX4 cells versus HEK293 cells. Each point is a unique peptide in the AV, MV, and secretome libraries. B , heat map showing selectivity of fold-change values of the six peptide candidates across the HEK293-MRGPRX families. C , MSA analysis and motif discovery with MEME Suite identifies conserved amino acids shared among the candidate hits ( left ). Structural alignment of the hits using AlphaFold ( right ). D , flow cytometric analysis of ERR1712142|105-166 cell binding. MRGPRX4-overexpressing cells and parental HEK293 cells were incubated with ERR1712142|105-166 fused with a FLAG-tag. Following incubation, the cells were stained with Alexa Fluor 647-labeled anti-FLAG antibodies to quantify the amount of specifically bound candidate ligand via flow cytometry. An Empty control, which expressed irrelevant recombinant protein, was included as an additional negative control to ensure the specificity of the binding measurement. E , flow cytometric analysis of cell binding of TFPI ( left ), SPINT2 ( middle ), and APP751 ( right ). MRGPRX4-overexpressing cells and parental HEK293 cells were incubated with TFPI, SPINT2, or APP751 fused with a His-tag. Following incubation, the cells were stained with Alexa Fluor 647-labeled anti-His antibodies. MFI, median fluorescence intensity. Data are shown as mean ± SD of 5 (in D ) or 3 (in E ) biological replicates. Statistical comparisons use two-tailed unpaired Student’s t tests. ns, nonsignificant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. F , dose-response curves of TFPI ( left ) and SPINT2 ( right ) in the presence of 200 μM UDCA using the MRGPRX4 PRESTO-Tango assay. Each point is one replicate. RLU, relative luminescence unit. G , dose-responses curves of UDCA in the presence of 0.2 μM TFPI ( left ) or 0.8 μM SPINT2 ( right ) in the MRGPRX4 PRESTO-Tango assay. Data are shown as mean ± SD of three technical replicates. MSA, multiple sequence alignment; SPINT2, serine peptidase inhibitor, Kunitz type 2; TFPI, tissue factor pathway inhibitor; UDCA, ursodeoxycholic acid.

Article Snippet: The cells were then incubated with ERR1712142 (fused with FLAG-tag), 30 nM TFPI (Acro Biosystems, Cat No. TFI-H5226, fused with His-tag), 30 nM SPINT2 (Sino Biological, Cat No. 10324-H08H, fused with His-tag) and 30 nM APP751 (a 751 amino-acid isoform of amyloid-beta precursor protein) (BioLegend, Cat No. 842601, fused with His-tag) prepared in 1% PBSA for 4 h at 4 °C on an end-to-end rotator.

Techniques: Binding Assay, Incubation, FLAG-tag, Staining, Labeling, Flow Cytometry, Control, Recombinant, Negative Control, Fluorescence, Two Tailed Test, Sequencing

Protein candidates identified from microarray gene expression analysis, listed by gene symbol, sorted by adjusted p value (p adj< 0.05; Benjamini-Hochberg adjustment).

Journal: Frontiers in Oncology

Article Title: Characterizing the secretome of EGFR mutant lung adenocarcinoma

doi: 10.3389/fonc.2023.1286821

Figure Lengend Snippet: Protein candidates identified from microarray gene expression analysis, listed by gene symbol, sorted by adjusted p value (p adj< 0.05; Benjamini-Hochberg adjustment).

Article Snippet: MDK and SPINT2 were quantified using the Human MDK ELISA Kit (Invitrogen, EH319RB) and SPINT2 (HAI-2) Human ELISA Kit (Invitrogen, EH319RB and EHSPINT2) according to kit instructions.

Techniques: Microarray, Expressing

Survival analysis of protein candidates identified from filtering pipeline. Overall patient survival was analyzed (log rank test, median split) for EGFR mutant tumors, n=125 and EGFR wild type tumors, n=68 from the NCBI GEO GSE 31219 dataset. (A) ENO1. (B) PFKP (C) RAC1 (D) SPINT2 (E) MDK. n.s. non-significant.

Journal: Frontiers in Oncology

Article Title: Characterizing the secretome of EGFR mutant lung adenocarcinoma

doi: 10.3389/fonc.2023.1286821

Figure Lengend Snippet: Survival analysis of protein candidates identified from filtering pipeline. Overall patient survival was analyzed (log rank test, median split) for EGFR mutant tumors, n=125 and EGFR wild type tumors, n=68 from the NCBI GEO GSE 31219 dataset. (A) ENO1. (B) PFKP (C) RAC1 (D) SPINT2 (E) MDK. n.s. non-significant.

Techniques: Mutagenesis

ELISA analysis of selected protein candidates in secretome conditioned media. Mean values ± SD are shown, experiment performed in technical duplicate (Student’s unpaired T-test, two tailed). (A) MDK (B) GDF15 (C) SPINT2. ** p < 0.01, *** p < 0.001, **** p < 0.0001, n.s. non-significant.

Journal: Frontiers in Oncology

Article Title: Characterizing the secretome of EGFR mutant lung adenocarcinoma

doi: 10.3389/fonc.2023.1286821

Figure Lengend Snippet: ELISA analysis of selected protein candidates in secretome conditioned media. Mean values ± SD are shown, experiment performed in technical duplicate (Student’s unpaired T-test, two tailed). (A) MDK (B) GDF15 (C) SPINT2. ** p < 0.01, *** p < 0.001, **** p < 0.0001, n.s. non-significant.

Techniques: Enzyme-linked Immunosorbent Assay, Two Tailed Test

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spint2 (Sino Biological)

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1) Product Images from "Molecular Display of the Animal Meta-Venome for Discovery of Novel Therapeutic Peptides"

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